TIGS
Tig Haus
Materials
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4 x Snapware 8-cup plastic food storage containers
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0.2 um filtered seawater (FSW)
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Tigriopus californicus
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Reed Mariculture Instant Algae Shellfish Diet 1800
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Plastic pipette
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Additional container
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Power drill w/ 3/16" drill bit
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Steps
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Drill 3/16" hole in corner of the container lids.
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For ventilation.
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About the size to allow an aquarium airline joint through, in case we want to run an airline in the future.
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Wash containers and ​lids. Tap water, no soap. Rinse with FSW.​
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Add 0.75 L FSW to each container.
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Add 0.5 mL of Shellfish Diet to each container and mix into FSW.
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Transfer ~100 mating pairs of tigs to additional container with FSW.
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Transfer step is to help prevent tiny nauplii from being transferred and to limit the algae being transferred to the Tig Haus.​
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Mating pairs chosen to help ensure reproduction will occur quickly and initial sex ratios are about even.
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Add 5 pairs at a time to Tig Hauses in a random order until reaching a total of 25 pairs per Haus.
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Add 20 males from original stock to transfer container to "wash."​
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This is to increase genetic diversity - the main tig culture was established from ~15 mating pairs from original stock.​
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Add 5 males to each Tig Haus in same random order.​
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Repeat with females from original stock.
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In a similar fashion, add 7 male and 8 female tigs from main stock to each Tig Haus.
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Total tigs per Tig Haus = 75
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Cover tigs, expose to ambient or 12/12-hr light-dark cycle.